A simplified method for computer analysis of autoradiograms from two-dimensional gels.

نویسندگان

  • J C Garrison
  • M L Johnson
چکیده

A simple method is described for computer analysis of a discrete number of spots on autoradiograms from two-dimensional gels. The method involves digitizing the density data on an autoradiograph with a rotating drum densitometer and displaying the data on a graphics computer terminal. The software allows the operator to select the boundaries of the spots to be analyzed from the terminal, then integrates the the density of the spots and tabulates the data. Graphics options allow the operator to display a computer-generated image of the area of the film being analyzed. Accurate integration of weak or overlapping spots is accomplished by a nonlinear least squares fit of the density data to normal Gaussian curves in the x and y dimensions followed by analytical integration of the equations. Since the software is written in Fortran IV and the equipment required to run the programs is available in most computing centers, this technique should allow laboratories of modest resources to quantitate information from two-dimensional gels.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Knee Kinematic Improvement After Total Knee Replacement Using a Simplified Quantitative Gait Analysis Method

Objectives: The aim of this study was to extract suitable spatiotemporal and kinematic parameters to determine how Total Knee Replacement (TKR) alters patients’ knee kinematics during gait, using a rapid and simplified quantitative two-dimensional gait analysis procedure. Methods: Two-dimensional kinematic gait pattern of 10 participants were collected before and after the TKR surgery,...

متن کامل

Simulation of Boiling in a Vertical Channel Using Ensemble Average Model

Simulation of turbulence boiling, generation of vapour and predication of its behaviour are still subject to debate in the two-phase flow area and they receive a high level of worldwide attention. In this study, a new arrangement of the three dimensional governing equations for turbulence two-phase flow with heat and mass transfer are derived by using ensemble averaging two-fluid model and ...

متن کامل

Identification of polypeptides on two-dimensional electrophoresis gels by amino acid composition.

We present a method that can, in principle, provide tentative identification of a substantial proportion of the polypeptides resolvable on a given two-dimensional electrophoresis gel. Computerized microdensitometry of autoradiograms from 20 gels labeled in turn with each of the 20 common amino acids provides the data for simultaneously measuring the amino acid composition of all polypeptides of...

متن کامل

Stress Analysis of the Human Ligamentous Lumber Spine-From Computer-Assisted Tomography to Finite Element Analysis

Detailed investigation on biomechanics of a complex structure such as the human lumbar spine requires the use of advanced computer-based technique for both the geometrical reconstruction and the stress analysis. In the present study, the computer-assisted tomography (CAT) and finite element method (FEM) are merged to perform detailed three dimensional nonlinear analysis of the human ligamentous...

متن کامل

Analysis of H-2 and Ia molecules by two-dimensional gel electrophoresis

Mouse lymphocyte H-2 and Ia glycoproteins have been analyzed with a two-dimensional (2-D) acrylamide gel electrophoresis technique, in which proteins are separated first according to their charge in isoelectrofocusing gels and then according to their size in sodium dodecyl sulfate gels. Individual polypeptide chains from radiolabeled cells are resolved as discrete spots on autoradiograms of the...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of biological chemistry

دوره 257 21  شماره 

صفحات  -

تاریخ انتشار 1982